{"hq_id":"hq-c-org-000587","name":"Indigotine (Indigo Carmine; FD&C Blue No. 2; E132)","context":"human_adult","timestamp":"2026-10-27T18:49:52.311Z","derived_by":{"label":"ALETHEIA-derived","engine":{"commit":"a1c6e861599cc34c5db0318ea1a7ac13a327e074","commit_source":"vercel_env","tree_clean":null},"params":{"sha256":"88ae43137e58ed0cd65588409efefee0d90253da01ef4459bf9ee25f888ec9a0","files":["axes.json","context-specificity.json","regulatory-mappings.json","synthesis-params.json","thresholds.json"]},"clock":{"year":2026},"inputs":[{"id":"hq-c-org-000587","sha256":"20b1c630dabd877263c840c3e2a01ac0e121f9b3557354efb34cb75212c0da72"}]},"risk_level":"low","risk_profile":{"magnitude":{"mean":0.2,"weightedMean":0.2,"std_dev":0,"confidence_interval_95":[0.2,0.2],"source_positions":[{"source":"HQ_DIRECT","year":2026,"value":0.2,"weight":0.35}]},"confidence":{"mean":0.5,"weightedMean":0.5,"std_dev":0,"confidence_interval_95":[0.5,0.5],"source_positions":[{"source":"HQ_DIRECT","year":2026,"value":0.5,"weight":0.35}]},"recency":{"mean":1,"weightedMean":1,"std_dev":0,"confidence_interval_95":[1,1],"source_positions":[{"source":"HQ_DIRECT","year":2026,"value":1,"weight":0.35}]},"methodology":{"mean":0.7,"weightedMean":0.7,"std_dev":0,"confidence_interval_95":[0.7,0.7],"source_positions":[{"source":"HQ_DIRECT","year":2026,"value":0.7,"weight":0.35}]},"specificity":{"mean":0.92,"weightedMean":0.92,"std_dev":0,"confidence_interval_95":[0.92,0.92],"source_positions":[{"source":"HQ_DIRECT","year":2026,"value":0.92,"weight":0.35}]},"consensus":{"exists":false,"strength":0,"disagreement_magnitude":0,"warning":"single_source_warning"}},"interpretation":{"summary":"Single source (HQ_DIRECT, 2026) indicates low risk.","confidence_note":"Evidence quality varies; interpretation requires caution.","context_note":null,"warning":"single_source_warning"},"sources":[{"id":"indigotine_fdc_blue2_e132_efsa_2014","type":"regulatory","title":"Indigotine Indigo Carmine FD&C Blue No 2 E132 CAS 860-22-0 CI Food Blue 1 Acid Blue 74 Disodium Indigo-5,5'-Disulfonate; EFSA 2014 ADI 5 mg/kg/day No Genotoxicity Ames Negative EFSA Journal 2014;12(7):3768; FDA ADI 5 mg/kg/day FD&C Blue No 2; NOT Southampton 2007 Hyperactivity Study No EU Warning Label; Permitted US EU Canada Australia NZ Japan; Medical Use IV 200mg Urological Surgery Ureteral Identification Hypotension Bradycardia; GI Absorption <1% Disulfonate Groups; No Azo Bond No Azo Reductase Metabolism; Indigo Chromophore Bicyclic Oxindole UV-Vis 610nm Blue; Sulfonated Indigo Parent Indigofera Tinctoria Natural; Synthetic Production Sulfonation Indigo CAS 482-89-3","year":2014},{"id":"r60-2026-09-28-d899-s3-b02-pmc-PMC10369292-efsa-2023-8103-indigo-carmine","type":"regulatory","title":"Follow‐up of the re‐evaluation of indigo carmine (E 132) as a food additive - PMC","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10369292/"},{"id":"r60-2026-09-28-d899-s4-b04-fr-1983-02-04-5251-5261-fdc-blue-2-pdftotext","type":"regulatory","title":"FD&C Blue No. 2","url":"https://archives.federalregister.gov/issue_slice/1983/2/4/5251-5261.pdf"},{"id":"r60-2026-10-02-d983-ac4-ctx-apis-data-domains","type":"regulatory","title":"Computational Toxicology and Exposure APIs: Data Domains (EPA CCTE)","url":"https://www.epa.gov/comptox-tools/computational-toxicology-and-exposure-apis-data-domains"},{"id":"r60-2026-10-02-d983-ac4-ctx-dashboard-release-notes","type":"regulatory","title":"CompTox Chemicals Dashboard: Release Notes (EPA)","url":"https://www.epa.gov/chemical-research/comptox-chemicals-dashboard-release-notes"},{"id":"echa-chem-reach-registrations","type":"regulatory","title":"ECHA CHEM, Download all substances: REACH registrations (ECHA's own list of REACH-registered substances)","year":2026,"url":"https://chem.echa.europa.eu/"},{"id":"pubchem-computed-cid-2723854","type":"database","title":"PubChem computed properties for CID 2723854 (computed, not measured; as recorded by PubChem)","year":2026,"url":"https://pubchem.ncbi.nlm.nih.gov/compound/2723854"},{"id":"doc-2026-10-03-d975-ecotox-ascii-09-15-2026","type":"database","title":"EPA ECOTOX Knowledgebase ASCII release ecotox_ascii_09_15_2026","url":"https://gaftp.epa.gov/ecotox/ecotox_ascii_09_15_2026.zip"},{"id":"r60-2026-09-30-d918-oehha-prop65-list-csv-own-url","type":"regulatory","title":"OEHHA Proposition 65 List of Carcinogens or Reproductive Toxicants (CSV, list date 31-Jul-26)","url":"https://oehha.ca.gov/sites/default/files/media/2025-01/p65chemicalslist.csv"},{"id":"r60-2026-10-02-d-955-s9-d955-s9-osha-table-z-1","type":"regulatory","title":"Permissible Exposure Limits – OSHA Annotated Table Z-1","url":"https://www.osha.gov/annotated-pels/table-z-1"},{"id":"ctx_pred_dtxsid1020190","type":"reference","title":"EPA CompTox CTX API: predicted properties for DTXSID1020190 (Indigotine (Indigo Carmine; FD&C Blue No. 2; E132))","url":"https://comptox.epa.gov/ctx-api/chemical/property/predicted/search/by-dtxsid/DTXSID1020190"},{"id":"pubchem-computed-structure-cid-2723854","type":"database","title":"PubChem computed structure descriptors for CID 2723854 (computed, not measured; as recorded by PubChem)","year":2026,"url":"https://pubchem.ncbi.nlm.nih.gov/compound/2723854"}],"sources_scope":"record","derived_markers":[],"warnings":["unmapped_classification:IARC:not evaluated by iarc for carcinogenicity — indigo","unmapped_classification:US EPA (CompTox genotoxicity summary):epa comptox computed genotoxicity summary: positiv","unmapped_classification:US EPA (CompTox genotoxicity summary):epa comptox computed genotoxicity summary: positiv","single_source_warning"],"meta":{"synthesis_version":"1.1.0","config_version":"1.1.0","n_sources":1,"db_version":44,"warnings":["unmapped_classification:IARC:not evaluated by iarc for carcinogenicity — indigo","unmapped_classification:US EPA (CompTox genotoxicity summary):epa comptox computed genotoxicity summary: positiv","unmapped_classification:US EPA (CompTox genotoxicity summary):epa comptox computed genotoxicity summary: positiv"]},"schema":"synthesis","_notice":"ALETHEIA output is reference data, not professional advice. Not a substitute for primary agency sources or qualified professionals. See https://aletheia.holisticquality.io/disclaimer.","_disclaimer_url":"https://aletheia.holisticquality.io/disclaimer","available_contexts":["human_adult","human_child","aquatic_life","human_teen","human_elderly","human_infant","human_pregnant"]}